Análisis Morfológico y Toxicológico de Cultivos de Pseudnitzchia Pungens Var Pungens (Bacillareophyceae)

SAR EUGENIA; ANDRINOLO DARIO; SUNESEN INES

BOLETíN DE LA SOCIEDAD ARGENTINA DE BOTáNICA

Año: 2006 vol. 41 p. 193 - 201

0373-580X

Summary: Morphological and toxicological analysis of cultures of Pseudo-nitzschia pungens var.Morphological and toxicological analysis of cultures of Pseudo-nitzschia pungens var. pungens (Bacillariophyceae). In the framework of a project of monitoring of harmful diatom species carried out in the Northern area of the Golfo San Matías (Provincia de Río Negro, Argentina) we found and established cultures of Pseudo-nitzschia pungens (Grunow ex. P.T. Cleve) Hasle var. pungens. The strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata carried out in the Northern area of the Golfo San Matías (Provincia de Río Negro, Argentina) we found and established cultures of Pseudo-nitzschia pungens (Grunow ex. P.T. Cleve) Hasle var. pungens. The strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata carried out in the Northern area of the Golfo San Matías (Provincia de Río Negro, Argentina) we found and established cultures of Pseudo-nitzschia pungens (Grunow ex. P.T. Cleve) Hasle var. pungens. The strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata carried out in the Northern area of the Golfo San Matías (Provincia de Río Negro, Argentina) we found and established cultures of Pseudo-nitzschia pungens (Grunow ex. P.T. Cleve) Hasle var. pungens. The strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata carried out in the Northern area of the Golfo San Matías (Provincia de Río Negro, Argentina) we found and established cultures of Pseudo-nitzschia pungens (Grunow ex. P.T. Cleve) Hasle var. pungens. The strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata (Bacillariophyceae). In the framework of a project of monitoring of harmful diatom species carried out in the Northern area of the Golfo San Matías (Provincia de Río Negro, Argentina) we found and established cultures of Pseudo-nitzschia pungens (Grunow ex. P.T. Cleve) Hasle var. pungens. The strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulata Pseudo-nitzschia pungens (Grunow ex. P.T. Cleve) Hasle var. pungens. The strains isolated from net phytoplankton samples obtained in Las Grutas and Piedras Coloradas, (Golfo San Matías) were cultured in F/2 medium, with silica added, and maintained at 20 ºC, under continuous light supplied by cool-white fluorescent tubes. Cultures were essayed for domoic acid (DA) by High Performance Liquid Chromatography (HPLC). Materials from field and cultures were studied with light and scanning electron microscopy. Comparison with closely related taxa, P. pungens var. cingulataP. pungens var. cingulata Villac and P. multiseries (Hasle) Hasle, and analysis of the fine morphology of cultured material, were conducted and discussed. In spite of P. pungens var. pungens has been reported as domoic acid producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. conducted and discussed. In spite of P. pungens var. pungens has been reported as domoic acid producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. conducted and discussed. In spite of P. pungens var. pungens has been reported as domoic acid producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. conducted and discussed. In spite of P. pungens var. pungens has been reported as domoic acid producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. conducted and discussed. In spite of P. pungens var. pungens has been reported as domoic acid producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. P. multiseries (Hasle) Hasle, and analysis of the fine morphology of cultured material, were conducted and discussed. In spite of P. pungens var. pungens has been reported as domoic acid producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. producer, the toxin was not detected in any of the cultures tested. P. pungens var. pungens has been reported as domoic acid producer, the toxin was not detected in any of the cultures tested. Key words: Pseudo-nitzschia pungens var. pungens, cultures, morphology, domoic acid.: Pseudo-nitzschia pungens var. pungens, cultures, morphology, domoic acid.