Lactobacillus casei CRL 431 added to a renutrition diet increases effectiveness to control the haemostatics alterations during an infectious process in malnourished mice

ZELAYA H; HARO C; LAIÑO J; ALVAREZ S; AGÜERO G

San Miguel de Tucumán. Tucumán. Argentina.

Simposio; III Simposio Internacional de II Bacterias Lácticas y Segundo Encuentro de la Red Argentina de Bacterias Lácticas (Red-BAL).; 2009

Centro de Referencia para Lactobacilos - CERELA- CONICET

The activation of the coagulation as a result of an infectious process is a well-known phenomenon that can be considered like part of the defenses of the host. Previously, we demonstrated that Lactobacillus casei CRL 431 administered by oral way exerts beneficial inmunomodulatory activity in inflammation-haemostasis process in a model of infection with Streptococcus pneumoniae (Sp) in immunocompetent mice. Considering that the malnourished mice showed increased susceptibility to pneumonia infection, the objective was to evaluate the effect of the oral administration of Lactobacillus casei CRL 431 (Lc) on the coagulation system, in a respiratory infection model in malnourished mice. For it, malnourished Swiss Albino mice (MNC) were used, which received during 7 days (d) balanced conventional diet (BCD) or BCD supplemented with Lc administered in the drink water (109 cells/day/mouse) during last 2d of the renutrición (BCD+Lc). Completed the same one, the groups BCD, BCD+Lc, MNC and wellnourished (WNC) were challenged intranasally with Sp. At 0, 12, 24, 120 and 240 hours post-infection (hpi) were determined in plasma and bronchoalveolar lavage (BAL): I-a)-Degree of activation of the coagulation by dosage of thrombin-antithrombin complexes (TATc). I-b)-Degree of inhibition of the coagulation by measuring inhibitor levels, such as Activated Protein C (APC) and Antithrombin (AT). II- To know which are the mechanisms of the coagulation mainly affected, we determined in plasma: Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Fibrinogen concentration (F) and Platelets count. The malnutrition altered all the evaluated parameters and only the diet supplemented with Lc allowed to normalize them (0 hpi). The infection induced in MNC increase of TATc and F, decrease of AT, late increase of APC and did not induce modification of platelets count. Whereas the mice that received the lactic bacterium during the renutrition period showed behavior similar to WNC relating to TATc (TATcplasma120hpi MNC=25,00±0,08, WNC=13,99±1,20, BCD=25,50±3,53, BCD+Lc=7,60±2,30µg/L), inhibitors (APC y AT) and platelets count. Also it was observed normalization of APTT and recovery of PT and F. On the basis of the obtained results it is possible to be concluded that the different renutrition diets showed different degree of effectiveness before infection. Also it was observed that BCD+Lc was effective to limit the activation of coagulation and normalize their regulating proteins. This effect would be beneficial for the host because although the coagulation system is considered part of the mechanisms of defense, its excessive activation can lead to an additional damage.