Differential effects of lactic acid bacteria peptidoglycans on lung epithelial cells and alveolar macrophages

FERRERO, MARIANA CRISTINA; ALVAREZ SUSANA; YANINA KOLLING; BALDI, PABLO CÉSAR; SALVA SUSANA

Buenos Aires

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2017

Sociedad Argentina de Inmunología

We demonstrated thatpeptidoglycan from Lactobacillus rhamnosus CRL1505 (Pg05) is aseffective as viable strain for improving systemic andrespiratory humoral immune response against Streptococcus pneumoniae in malnourished mice.These immunomodulatory capacity cannot beextrapolated to peptidoglycans (Pgs) from other strains. Theaim of this work was to study the interactions of Pgs obtained fromdifferent lactobacilli, with mice lung epithelial cells (LEC) or alveolarmacrophages (AM), in order to elucidate the mechanisms involvedin the differential effect of Pg05. We compared Pg05 with Pgs from thenon-probiotic L. rhamnosus CRL534 (Pg534) and from theprobiotic L. plantarum CRL1506 (Pg06). AM or LEC were cultured24h or 24-48h with different concentrations of Pg05, Pg06 or Pg534, inthe absence or presence of S. pneumoniae. In addition, we studiedthe cytokines production of LEC with conditioned medium fromAM pre-stimulated with Pgs. The results showed that 90% of LECand AM remained viable after the treatments with Pgs. Pg05 (50ug/mL) and Pg06 were able to differentially modulate the cytokinesproduction in AM and LEC. Unlike other Pgs, Pg05significantly modulated the activation of AM by an increase of IL-1β, KC and IL-10 with adecrease of TNF-α (p