Effect of immunobiotic bifidobacteria on the innate antiviral immune response in bovine intestinal epithelial cells: immunotranscriptomic analysis

LEONARDO ALBARRACÍN; NANA SATO; HISAKAZU KOBAYASHI; TOMONORI NOCHI; WAKAKO IKEDA-OHTSUBO; JIN-ZHONG XIAO; NORIYUKI IWABUCHI; HARUKI KITAZAWA; JULIO VILLENA; AKM HUMAYUN KOBER; PAULRAJ KANMANI; HISASHI ASO; TADAO SAITO

Valencia

Congreso; FEMS 2017 - 7th Congress of European Microbiologists; 2017

FEMS

EFFECT OF IMMUNOBIOTIC BIFIDOBACTERIA ON THE INNATE ANTIVIRAL IMMUNERESPONSE IN BOVINE INTESTINAL EPITHELIAL CELLS: IMMUNOTRANSCRIPTOMICANALYSISL. Albarracín1, A.K.M.H. Kober2, H. Kobayashi2, N. Sato2, K. Paulraj2, W. Ikeda-Ohtsubo2, T. Nochi2,H. Aso2, N. Iwabuchi3, J.Z. Xiao3, T. Saito2, J. Villena1, H. Kitazawa21CENTRO DE REFERENCIA PARA LACTOBACILOS CERELA-CONICET,Laboratory of Immunobiotechnology, San Miguel de Tucumán, Argentina2Tohoku University, Graduate School of Agricultural Science, Sendai, Japan3Morinaga Milk Industry Co.- Ltd., Food Science and Technology Institute, Kanagawa, JapanBackgroundsPrevious studies showed that Bifidobacterium infantis strain or B. breve strain significantly reduced rotavirus titers in infected bovine intestinal epithelial (BIE) cells.ObjectivesIn this work, transcriptomic studies in BIE cells were performed to advance in the understanding the mechanisms involved in the antiviral activity of bifidobacteria.MethodsBIE cells were stimulated with B. infantis or B. breve (5 x 108 cells/ml) and then challenged with the TLR3 agonist poly(I:C). BIE cells with no bifidobacteria treatment were used as controls. The transcriptomic response was evaluated 12 h after poly(I:C) challenge using the Bovine (G3) Gene Expression Microarray (Agilent). Variations in transcript abundance with a t-test p