Effect of Lactobacillus fermentum CRL1446 on immune-metabolic parameters and intestinal microbiota composition in obesity in high-fat diet fed mice.

FABERSANI, E; ABEIJÓN MUKDSI, C; ROSS, GR; GAUFFIN CANO, PAOLA; MARQUEZ, A; MEDINA, R

Buenos Aires

Congreso; IUNS 21ST INTERNATIONAL CONGRESS OF NUTRITION (ICN); 2017

Background and objectives: Dietary strategies, includingthe use of probiotics as preventive agents that handle the intestinalmicrobiota and/or modulate the function of adipose tissue, wouldbe suitable tools for the prevention or treatment of different conditions associated with obesity. In this context, our aim was to evaluatethe effect of Lactobacillus fermentum CRL1446 (CRL1446) onmetabolic and immunological parameters and on the compositionof intestinal microbiota in a model of obesity induced by a high-fatdiet (HFD).Methods: Balb/c mice were separated into the followinggroups: 1) SD: Standard diet, 2) HFD: High-fat diet, 3) HFD+CRL1446: mice receiving a HFD and a daily dose of 1 x 108CFU of L. fermentum CRL1446 (CRL1446) in the drinking water.The dietary intervention was maintained for 6 weeks. Weight andfood intake were monitored daily. It were determined at 20 and 45days of experimental period: fat tissue weight (histologic studies),metabolic parameters (triglyceride (TG), total cholesterol (TC),glucose (Glu) and leptin (Lep)), seric immunologic parameters(TNF-alpha, IL-6, IL10 and MCP-1), macrophage and adipocytefunctionality (ex vivo studies) and the gut microbiota composition(evaluated by high-throughput sequencing of 16S rRNA geneamplicons).Results: CRL1446 strain induces a decrease in body and adiposetissue weights and the size of adipocytes. A decrease in plasmaticglucose, cholesterol, triglycerides, leptin, TNF-alpha andIL-6 levels and an increase of IL-10 were observed. Ex vivo studiesshowed a minor secretion of leptin by adipocytes. In macrophages,CRL1446 induced lower levels of MCP-1, TNF-alpha, IL-6 andhigher levels of IL-10. Regarding intestinal microbiota, an increasein Lactobacillus and a decrease in Firmicutes/Bacteroidetes (F/B)index were observed.Conclusions: The supplementation with L. fermentumCRL1446 produce changes in intestinal microbiota structure andmetabolic and immunologic parameters. CRL1446 could potentiallybe a probiotic strain useful to correct the immuno-metabolicalterations associated with obesity through its adipomodulatorycapacity.