The respiratory commensal bacteria Corynebacterium pseudodiphtheriticum 090104 improves respiratory antiviral innate immunity

HARUKI KITAZAWA; SUSANA SALVA; CECILIA RODRIGUEZ; TADA, ASUKA; VYACHESLAV MELNIKOV; FERNANDA RAYA-TONETTI; PAULRAJ KANMANI; PATRICIA CLUA, ; JULIO VILLENA; ALVAREZ, SUSANA; HIKARU IIDA; VIZOSO-PINTO, MARIA G.; HUMAYUN KOBER AKM

Valencia

Congreso; 7th Congress of the Federation of European Microbiological Societies (FEMS) and 26th Congress of the Spanish Society for Microbiology (SEM); 2017

The nasal administration of Corynebacterium pseudodiphtheriticum strain 090104 increases the elimination of Staphylococcus aureus from the nasal cavity in volunteers. The ability of the 090104 strain to improve respiratory immunity or its capacity to increase resistance against respiratory virus were not investigated before. Therefore, this work examined the effect of viable (Cb) or heat-killed (HkCb) C. pseudodiphtheriticum 090104 on the innate respiratory antiviral immune response and the resistance against influenza infection in mice. Adult Balb/c mice were treated with 108 cells of Cb or HkCb by the nasal route during two consecutive days. Treated and untreated control mice were then nasally challenged with influenza virus. Lung tissue damage and respiratory and systemic immune responses were studied at several time points after viral challenge. Cb treatment protected mice by reducing pulmonary injury and lung viral loads. This effect was associated to a differential regulation of the production of antiviral factors since respiratory levels of IFNs were enhanced (p