EFFECT OF PROBIOTIC ADMINISTRATION ON BIOCHEMICAL PARAMETERS AND INTESTINAL MICROBIOTA IN CALORIC RESTRICTION

FABERSANI, E; RUSSO, M; ROSS, G.R.; MEDINA, R; GAUFFIN CANO, P

Mar del Plata

Jornada; LI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2015

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

Effects ofcaloric restriction diet and Lactobacillus fermentum (Lf) CRL1446 and L.casei (Lc) CRL431 (probiotic strains) administration on metabolicparameters and intestinal microbiota was studied. Balb/c mice were divided intothe following groups: normal diet (ND), caloric restriction diet (CR), CR dietplus Lf and CR plus Lc. CR diet was administered during 45 days and strainswere given in the dose of 10 _ cells/mL/day/mouse. Triglyceride (TG), total cholesterol(TC), glucose and leptin were determined in plasma. Gut microbiota wasevaluated by high-throughput sequencing of 16S rRNA gene amplicons. Glucose,TC, TG and leptin levels were decreased in CR groups. Lc administrationincreased glucose and leptin levels. Lf decrease TC and leptin levels, showinglower values than in CR group. Gut microbiota studies, showed that CR dietincreased abundance of Bifidobacterium and Lactobacillus genus and improvedBacteroidetes:Firmicutes ratio. Lc administration increased abundance ofLactobacillus genus. However, Lf administration increased abundance ofBifidobacterium genus and was able to maintain Lactobacillus genus during timeof treatment. Probiotic strains improved metabolic parameters modulatingintestinal microbiota differently. Lc and Lf increased dominance of certainspecies reducing biodiversity. L.  fermentum exerted a bifidogenic effectunder CR conditions.