CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
capítulos de libros
Título:
Culture media formulation for metabolic studies of lactic acid bacteria
Autor/es:
FABIANA M. SAGUIR AND MARÍA C. MANCA DE NADRA
Libro:
Nitrogen Compounds Metabolism by Lactic Acid Bacteria
Editorial:
Transworld Research Network
Referencias:
Lugar: Kerala, India; Año: 2008; p. 2 - 23
Resumen:
Due to nutritional complexity of lactic acid
bacteria (LAB), they are frequently cultivated in
complex or natural media containing energy sources,
precursors and stimulatory substances for cell growth
and division. However the complexity of these media
such as MRS o M17 cannot be used to characterize
precisely the nutritional requirements of LAB.
Moreover the consumption of the various
intermediary metabolites for biosynthesis of
macromolecules via the anabolic pathways cannot be
correctly established when using complex organic compounds, because they
provoke considerable uncertain in these studies. Synthetic media sufficiently
simple to allow full carbon balance is therefore an essential step in the study of
nitrogen metabolism of LAB. The use of a chemically defined medium is also
important to enhance the proteinase activity by termophilic lactobacilli and to
study the regulation of this enzyme. Different investigations concerning to
development of synthetic media for an optimal LAB growth have been carried
out. A simple synthetic medium was development for sustained growth of
Lactococcus lactis for at least 50 generations of cell division. In this medium
growth was not perturbed by the suppression of the ammonium ion component.
This fact indicates that the limited number of amino acids retained in the
simplified defined medium could supply the entire nitrogen requirement of the
cell. Recently a chemically defined medium for lactobacilli growth was
improved mainly of the basis of annulling limitations with respect to amino
acids. In addition increments of some vitamins produce the best bacterial
growth in the formulated medium, for the sustained growth of Lactobacillus
plantarum strains. This medium is proposed for conducting metabolic and
genetics studies of LAB.
correctly established when using complex organic compounds, because they
provoke considerable uncertain in these studies. Synthetic media sufficiently
simple to allow full carbon balance is therefore an essential step in the study of
nitrogen metabolism of LAB. The use of a chemically defined medium is also
important to enhance the proteinase activity by termophilic lactobacilli and to
study the regulation of this enzyme. Different investigations concerning to
development of synthetic media for an optimal LAB growth have been carried
out. A simple synthetic medium was development for sustained growth of
Lactococcus lactis for at least 50 generations of cell division. In this medium
growth was not perturbed by the suppression of the ammonium ion component.
This fact indicates that the limited number of amino acids retained in the
simplified defined medium could supply the entire nitrogen requirement of the
cell. Recently a chemically defined medium for lactobacilli growth was
improved mainly of the basis of annulling limitations with respect to amino
acids. In addition increments of some vitamins produce the best bacterial
growth in the formulated medium, for the sustained growth of Lactobacillus
plantarum strains. This medium is proposed for conducting metabolic and
genetics studies of LAB.