Genes and proteins regulation during Listeria monocytogenes FBUNT biofilm formation at 10 ºC in response to lactocin AL705

MELIAN, C.; CASTELLANO P.; BETAECOURT, E; SEGLI, F.; MENDOZA, L.M.; VIGNOLO, G.

Haifa

Conferencia; 34th EFFoST International Conference 2020; 2020

Technion-Israel

Listeria monocytogenes is a foodborne pathogen is able to survive and multiply under different stress conditions. The high persistence in industrial premises and foods of the pathogen is due to the ability to form biofilm. Strategies to overcome L. monocytogenes persistence consist in inhibition of biofilm formation or removal of mature biofilms through antimicrobial compounds like bacteriocin. The aim of this work was analyze L. monocytogenes FBUNT genes and proteins regulated during biofilm formation at 10 °C exposed or not to lactocin AL705 by using transcriptional and comparative proteomic approach. Listeria was growth in culture media with presence or absence of lactocin AL705 (at subinhibitory concentration) during 6 days at 10°C. Sessile cells were growth in 24 wells microplates and planktonic cells in tubes 3ml. Genes related to aggregation, adhesion, virulence, biofilm formation and stress factors were evaluated by PCR and RT-qPCR. Respect to control planktonic cells lmo1601, lmo1634, lmo2016, agrA were up-regulated in biofilm cells but the bacteriocin addition caused a decreased their expression. luxS, agrB, agrD and lmo0327 were up-regulated in presence of bacteriocin. Moreover, L. monocytogenes FBUNT proteome under planktonic, lactocin AL705-treated and untreated biofilms were analyzed by nanoLC-MS/MS. Compared to planktonic cells, sessile cells involved protein abundance shifts associated with nucleic acids metabolism (Lmo0935, RsmH), lipids metabolism (Lmo2089, GlpD) and transport (Lmo1875, Lmo2372). When sessile cells were treated with lactocin AL705, proteins up-regulation were mostly related to carbohydrate metabolism and nutrient transport. Notably, transport systems as β-glucosidase IIABC (lmo0027), cellobiose (lmo2763) and trehalose (lmo1255) specific PTS proteins were highly overexpressed. Contrary, mannose (lmo0098) specific PTS protein was down-regulated. The results indicate that a sublethal dose lactocin AL705 induced adaptation mechanisms in L. monocytogenes FBUNT sessile cells at 10 ºC, which would provide valuable data to know specific genes targeting to control L. monocytogenes biofilm upon the treatment with bacteriocins.