Andean tubers fermentation with potentially probiotic folate-producing lactic Acid Bacteria.

MOSSO, A.L. ; VIGNOLO, G.; LEBLANC, J-G.; SAMMAN, N.

Tucuman

Simposio; V International Symposium on Lactic Acid Bacteria (SIBAL). Benefitting from lactic Acid Bacteria. Progress in Health and Food.; 2016

CERELA-CONICET

lF"mrate is a B-group vitamin that cannot be synthesized by humans nutained' and must be exogenously lt is known that certain strains of lactic acid bacterir irnal can produce natural fo- llm'es which, unlike the chemically synthesized form folic acid, .r.- not associated w¡th certain uum'r''anted side effects.Eres can confer .Fermentaüon of vegetables with seiected potentially probioüc cul- health benefits to the host since these could produce beneficial metabolitesY: 'n::te:s:ü"n1al: cl:o:n^ditti-o!n:s: :(Tsc,rc:)l io*f: f:o:l:alte -spturoddv uwcainsg t o evaruate ih. tor",.n.e to simurated gas- LAB strains .n¿ ,r"1,"-r;ñ il;;T;; roean tubers in order to obtain potenüalty probiotic products with elevated folate levels. e strains of LAB -Loctobaciilus (1.) sokei2T2MM10 and 2T3MSS , L. fermentumT3M3, L. ca_:':dlu:cyt :nja.mye"dt lT:oyc:ois-h . yT"h.1e:y ow.elsrien 'cilhva irsaocrateter.idz erdlo mas a t'ad¡t'-;;;í;ndean rermented potato being rorr." proir;;;;','n.j;lr,""o :est their tolerance to sGlc' The rn vitro analyzes deteimined tlat the five strains coutd -¡ive Ín gastríc juíce (2.5h, pH 2.5, containing 0.3% of p"prin) w¡trrout any reducüon in I counts' However, they showed lower resistance to int"rtin.l conditions (2.5h, pH g.o, rtaining o'3% of bile salts and o'o3% of pancreaün) with the excepüon of L. coser3T3Ms2, r':se survivability was not affected. These strains were used to ferment three Andean tu- s (Solonum tuberosum ssp andigeno collareja potato variety, oxotis tuberosa purple oca ety and ullucus tuberosum pink papalisa variety). sterile .oát.¿ purees were inoculated ic samples were collected at 0, 6, 24 hours and 2g days to determine cell growth, pH and pl folate content. Although all five strains were able to grow and produce folates in the ree tubers, L' sokei2T2MM10 showed the hilhest increasá after 24hincubaüon in oca which rep_ pnts a two and threefold increase compared to the unfermentéd-tubers. Folate concentra- rincollarejapotato,whichwasfermentedfor24hwith L.sakei2T3MSgstrain, was7g2 g' thus represenüng a 265% increase in comparison with the unfermented potato. viable | :ounts of all strains remain stabre (>rog g uFc/cl for 2gory, or rior.ge at 4"c. concrusion:É adequate selection of LAB could be used to produce new tuber-based products with el- aled folate concentrations.