INVESTIGADORES
VIGNOLO Graciela Margarita
artículos
Título:
Hydrolysis of pork muscle sarcoplasmic proteins by Lactobacillus curvatus and Lactobacillus sake
Autor/es:
S FADDA,; Y SANZ,; G VIGNOLO,; MC ARISTOY,; G OLIVER,; F TOLDRA,; GRACIELA MARGARITA VIGNOLO
Revista:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Editorial:
American Society for Microbiology
Referencias:
Lugar: Washington; Año: 1999 vol. 65 p. 578 - 584
ISSN:
0099-2240
Resumen:
Lactobacillus curvatus CECT 904 and Lactobacillus sake CECT 4808 were selected on the basis of their
proteolytic activities against synthetic substrates. Further, the effects of whole cells, cell extracts, and a
combination of both enzymatic sources on muscle sarcoplasmic proteins were determined by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography analyses.
Strains of both species displayed proteinase activities on five sarcoplasmic proteins. The inoculation of
whole cells caused a degradation of peptides, whereas the addition of cell extracts resulted in the generation
of both hydrophilic and hydrophobic peptides. This phenomenon was remarkably more pronounced when L.
curvatus was involved. Whole cells also consumed a great amount of free amino acids, while the addition of
intracellular enzymes contributed to their generation. L. sake accounted for a greater release of free amino
acids. In general, cell viability and also proteolytic events were promoted when cell suspensions were provided
with cell extracts as an extra source of enzymes.CECT 904 and Lactobacillus sake CECT 4808 were selected on the basis of their
proteolytic activities against synthetic substrates. Further, the effects of whole cells, cell extracts, and a
combination of both enzymatic sources on muscle sarcoplasmic proteins were determined by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography analyses.
Strains of both species displayed proteinase activities on five sarcoplasmic proteins. The inoculation of
whole cells caused a degradation of peptides, whereas the addition of cell extracts resulted in the generation
of both hydrophilic and hydrophobic peptides. This phenomenon was remarkably more pronounced when L.
curvatus was involved. Whole cells also consumed a great amount of free amino acids, while the addition of
intracellular enzymes contributed to their generation. L. sake accounted for a greater release of free amino
acids. In general, cell viability and also proteolytic events were promoted when cell suspensions were provided
with cell extracts as an extra source of enzymes.L.
curvatus was involved. Whole cells also consumed a great amount of free amino acids, while the addition of
intracellular enzymes contributed to their generation. L. sake accounted for a greater release of free amino
acids. In general, cell viability and also proteolytic events were promoted when cell suspensions were provided
with cell extracts as an extra source of enzymes.was involved. Whole cells also consumed a great amount of free amino acids, while the addition of
intracellular enzymes contributed to their generation. L. sake accounted for a greater release of free amino
acids. In general, cell viability and also proteolytic events were promoted when cell suspensions were provided
with cell extracts as an extra source of enzymes.L. sake accounted for a greater release of free amino
acids. In general, cell viability and also proteolytic events were promoted when cell suspensions were provided
with cell extracts as an extra source of enzymes.
