Effect of riboflavin-producing bacteria against chemically-induced colitis in mice

LEVIT, R.; SAVOY DE GIORI, GRACIELA; DE MORENO DE LEBLANC, A.; LEBLANC, JEAN GUY

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2018 vol. 124 p. 232 - 240

1364-5072

Aim. To assess the anti-inflammatory effect associated to individual probiotic suspensions of riboflavin-producing lactic acid bacteria (LAB). In a colitis murine model.Methods and Results. Mice intrarectally inoculated with trinitrobenzene sulfonic acid (TNBS) were orally administered with individual suspensions of riboflavin-producing strains: Lactobacillus (Lact.) plantarum CRL2130, Lact. paracasei CRL76, Lact. bulgaricus CRL871, Streptococcus thermophilus CRL803; a non riboflavin-producing strain or commercial riboflavin. The extent of colonic damage and inflammation and microbial translocation to liver were evaluated.. iNOs enzyme was analyzed in the intestinal tissues and cytokine concentrations in the intestinal fluids. Animals given either one of the four riboflavin-producing strains showed lower macroscopic and histologic damage scores, lower microbial translocation to liver, significant decreases of iNOs+ cells in their large intestines and decreased pro-inflammatory cytokines, compared to mice without treatment. The administration of pure riboflavin showed similar benefits. Lact. paracasei CRL76 accompanied its anti-inflammatory effect with increased IL-10 levels demonstrating other beneficial properties in addition to the vitamin production. Conclusion. Administration of riboflavin-producing strains prevented the intestinal damage induced by TNBS in mice.Significance and impact of the study. Riboflavin-producing phenotype in LAB represents a potent tool to select them for preventing/ treating IBD.