Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A antigen Increases INF-γ, IL-6, and TNF-α cytokines after Intranasal Immunization

MANCHA-AGRESTI, P.; CASTRO, C.P; DOS SANTOS, J.S.; ARAUJO, M.A.; PEREIRA, V.B.; JEAN GUY LEBLANC; LECLERCQ, S.; AZEVEDO, V.

Frontiers in Microbiology

Frontiers Editorial Office

Lugar: Lausanne; Año: 2017

Using bacteria as vehicles for the delivery of vaccine plasmids is a promising vaccination strategy. In this sense, Lactococcus (L.) lactis has previously been modified in order to efficiently dilever DNA vaccines to host cells. In this study, an engineered invasive L. lactis strain expressing Fibronectin Binding Protein A (FnBPA+) from Staphylococcus aureus was used to deliver a new DNA vaccine against tuberculosis. The open reading frame (ORF) corresponding to Ag85A of Mycobacterium tuberculosis was cloned into the pValac vector (Vaccination using Lactic Acid bacteria). L. lactis FnBPA+ (pValac:Ag85A) was obtained, used for intranasal immunization of C57BL/6 mice and the immune response profile was evaluated. A significant increase in pro-inflammatory cytokines (IFN-γ, TNF-α and IL-6) was observed in the stimulated spleen cell supernatants, showing a systemic T helper 1 (Th1) cell response. L. lactis FnBPA+ (pValac:Ag85A) also induced a significant increase in antibody production (IgG and sIgA) in the bronchus as well as a significant increase of IgA in blodd serum of mice. These results show that the use of L. lactis FnBPA+ could be a good tool for the development of novel anti-TB DNA vaccine.