Factors stimulating riboflavin produced by Lactobacillus plantarum CRL 725 grown in a semi-defined medium

JUAREZ DEL VALLE, M.; LAIÑO, J. E.; SAVOY DE GIORI, G.; LEBLANC, JEAN GUY

JOURNAL OF BASIC MICROBIOLOGY

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2017 vol. 57 p. 245 - 252

0233-111X

Riboflavin (vitamin B2) is one of the B-group water-soluble vitamins and is essential for energy metabolism of the cell. The aim of this study was to determine factors that affect riboflavin production by Lactobacillus (L.) plantarum CRL 725 grown in a semi defined medium and evaluate the expression of its rib genes. The factors found to enhance riboflavin production in this medium was incubation at 30ºC, and the addition of specific medium constituents, such as casamino acids (10 g/l), guanosine (0.04 g/l) and sucrose as carbon source (20 g/l). In these conditions, the highest riboflavin concentrations were directly associated with significant increases in the expression of ribA, ribB and ribC genes. The culture conditions defined in this work and its application to a roseoflavin resistant mutant of L. plantarum allowed for a 6 fold increase in riboflavin concentrations in our semi-defined medium which were also significantly higher than those obtained previously using the same strain to ferment soymilk. These conditions should thus be evaluated to increase vitamin production in fermented foods.