Safety, functional properties and technological performance in whey-based media of probiotic candidates from human breast milk

BOCKELMANN, WILHELM; VINDEROLA, GABRIEL; ZACARÍAS, MARÍA FLORENCIA; REINHEIMER, JORGE; BINETTI, ANA; HELLER, KNUT

INTERNATIONAL MICROBIOLOGY

VIGUERA EDITORES

Lugar: Barcelona; Año: 2018

1139-6709

We aimed at isolating and characterising microorganisms present in human breast milk with probiotic potential. In an 8-weekpostpartum sampling period, two strains of bifidobacteria (Bifidobacterium longum LM7a and Bifidobacterium dentium LM8a?)and four strains of lactobacilli were isolated, all during the first 4-week postpartum. B. longum LM7a and B. dentium LM8a?,together with four strains previously isolated from breast milk (Bifidobacterium lactis INL1, INL2, INL4 and INL5), wereconsidered for further studies. Susceptibility of the strains to tetracycline, erythromycin, clindamycin, streptomycin, vancomycinand chloramphenicol was evaluated and the isolates exhibited, in general, the same properties as previously reported forbifidobacteria. All isolates showed low hydrophobicity and B. lactis and B. longum strains had satisfactory resistance to gastricdigestion and bile shock, but not to pancreatin. B. lactis INL1, B. longum LM7a and B. dentium LM8a? were selected for somecomparative technological studies. In particular, B. lactis INL1 displayed technological potential, with satisfactory growth incheese whey-based media in biofermentor and resistance to freeze-drying, accelerated storage conditions and simulated gastricdigestion