Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity.

TRUCCO, V.; REINHEIMER, J.; QUIBERONI A.; SUÁREZ, V.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2011 vol. 110 p. 935 - 942

1364-5072

Aims: The aim of this work was to study the adsorption step of two new temperate bacteriophages (Cb1 ⁄ 204 and Cb1 ⁄ 342) of Lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. Methods and Results: The effect of divalent cations, pH, temperature and cell viability on adsorption step was analysed. The Ca2+ presence was necessary for the phage Cb1 ⁄ 342 but not for the phage Cb1 ⁄ 204. Both phages showed to be stable at pH values between 3 and 8. Their adsorption rates decreased considerably at pH 8 but remained high at acid pH values. The optimum temperatures for the adsorption step were between 30 and 40C. For the phage Cb1 ⁄ 342, nonviable cells adsorbed a lower quantity of phage particles in comparison with the viable ones, a fact that could be linked to disorganization of phage receptor sites and ⁄ or to the physiological cellular state. The isolation of phage-resistant derivatives with good technological properties from the sensitive strains and their relationship with the cell heterogeneity of the strains were also made. Conclusions: Characterization of the adsorption step for the first temperate Lact. delbrueckii phages isolated in Argentina was made, and phage-resistant derivatives of their host strains were obtained. Significance and Impact of the Study: Some phage-resistant derivatives isolated exhibited good technological properties with the prospective to be used at industrial level.