Safety, functional properties and technological performance in whey-based media of probiotic candidates from human breast milk.

ZACARÍAS, M. F.; BINETTI, A. G.; BOCKELMANN, W.; REINHEIMER, J. A.; HELLER, K.; VINDEROLA, C. G.

INTERNATIONAL MICROBIOLOGY

VIGUERA EDITORES

Lugar: Barcelona; Año: 2018

1139-6709

We aimed at isolating and characterising microorganisms present in human breast milk with probiotic potential. In an 8-week13 postpartum sampling period, two strains of bifidobacteria (Bifidobacterium longum LM7a and Bifidobacterium dentium LM8a?)14 and four strains of lactobacilli were isolated, all during the first 4-week postpartum. B. longum LM7a and B. dentium LM8a?,15 together with four strains previously isolated from breast milk (Bifidobacterium lactis INL1, INL2, INL4 and INL5), were16 considered for further studies. Susceptibility of the strains to tetracycline, erythromycin, clindamycin, streptomycin, vancomycin17 and chloramphenicol was evaluated and the isolates exhibited, in general, the same properties as previously reported for18 bifidobacteria. All isolates showed low hydrophobicity and B. lactis and B. longum strains had satisfactory resistance to gastric19 digestion and bile shock, but not to pancreatin. B. lactis INL1, B. longum LM7a and B. dentium LM8a? were selected for some20 comparative technological studies. In particular, B. lactis INL1 displayed technological potential, with satisfactory growth in21 cheese whey-based media in biofermentor and resistance to freeze-drying, accelerated storage conditions and simulated gastric22 digestion.