The impact of long term grain feeding on glycolytic metabolism of cattle

PIGHIN, D.G.; WARNER, ROBYN D.; JACOB, R.; BEATTY, D.; NATHTHARAMPATHA, A.; FERGUSON, DREWE M.

Ghent

Congreso; 57 th International Congress of Meat Science and Technology-ICoMST; 2011

The aim of the work was to study the effect of long term grain feeding on the glycolytic metabolism of cattle. Eighteen 13-month old Bos-taurus Angus steers (369±14kg) were randomly assigned to different diets: grain based feedlot ration during 300 days (300GD); pasture during 150 days and then feedlot grain based ration for 150 days (150GD); and pasture during 300 days (300PD). Blood samples were collected at exsanguination for analysis of glucose, lactic acid and insulin concentrations. Muscle samples were removed from Psoas-major (PM) at 1, 2, 4 and 24h post-slaughter for glycogen and lactic acid levels assay. The ultimate pH of PM was taken at 24h post-mortem. All animals presented hyperglycemia at slaughter. High energy-fed animals (300GD and 150GD) showed higher levels of both glucose and lactic acid (p>0.05) in the plasma at slaughter. Plasma insulin was slightly increased in animals fed with high energy diet, for either 300d or 150d. No significant differences could be found in glycogen or in lactate levels between treatments. Animals fed high energy ration (300GD and 150GD) displayed higher rates of anaerobic utilization of glycogen during the first 4h, as evidenced by differences among diets during the first 2h (slopes: -17.3 and -26.0 for 300GD and 150GD vs.-10.6 for 300PD). Results give evidence of apparent impaired peripheric insulin sensitivity associated with high energy grainbased feeding, either during 300d or 150d. Post mortemBos-taurus Angus steers (369±14kg) were randomly assigned to different diets: grain based feedlot ration during 300 days (300GD); pasture during 150 days and then feedlot grain based ration for 150 days (150GD); and pasture during 300 days (300PD). Blood samples were collected at exsanguination for analysis of glucose, lactic acid and insulin concentrations. Muscle samples were removed from Psoas-major (PM) at 1, 2, 4 and 24h post-slaughter for glycogen and lactic acid levels assay. The ultimate pH of PM was taken at 24h post-mortem. All animals presented hyperglycemia at slaughter. High energy-fed animals (300GD and 150GD) showed higher levels of both glucose and lactic acid (p>0.05) in the plasma at slaughter. Plasma insulin was slightly increased in animals fed with high energy diet, for either 300d or 150d. No significant differences could be found in glycogen or in lactate levels between treatments. Animals fed high energy ration (300GD and 150GD) displayed higher rates of anaerobic utilization of glycogen during the first 4h, as evidenced by differences among diets during the first 2h (slopes: -17.3 and -26.0 for 300GD and 150GD vs.-10.6 for 300PD). Results give evidence of apparent impaired peripheric insulin sensitivity associated with high energy grainbased feeding, either during 300d or 150d. Post mortemPsoas-major (PM) at 1, 2, 4 and 24h post-slaughter for glycogen and lactic acid levels assay. The ultimate pH of PM was taken at 24h post-mortem. All animals presented hyperglycemia at slaughter. High energy-fed animals (300GD and 150GD) showed higher levels of both glucose and lactic acid (p>0.05) in the plasma at slaughter. Plasma insulin was slightly increased in animals fed with high energy diet, for either 300d or 150d. No significant differences could be found in glycogen or in lactate levels between treatments. Animals fed high energy ration (300GD and 150GD) displayed higher rates of anaerobic utilization of glycogen during the first 4h, as evidenced by differences among diets during the first 2h (slopes: -17.3 and -26.0 for 300GD and 150GD vs.-10.6 for 300PD). Results give evidence of apparent impaired peripheric insulin sensitivity associated with high energy grainbased feeding, either during 300d or 150d. Post mortemPM was taken at 24h post-mortem. All animals presented hyperglycemia at slaughter. High energy-fed animals (300GD and 150GD) showed higher levels of both glucose and lactic acid (p>0.05) in the plasma at slaughter. Plasma insulin was slightly increased in animals fed with high energy diet, for either 300d or 150d. No significant differences could be found in glycogen or in lactate levels between treatments. Animals fed high energy ration (300GD and 150GD) displayed higher rates of anaerobic utilization of glycogen during the first 4h, as evidenced by differences among diets during the first 2h (slopes: -17.3 and -26.0 for 300GD and 150GD vs.-10.6 for 300PD). Results give evidence of apparent impaired peripheric insulin sensitivity associated with high energy grainbased feeding, either during 300d or 150d. Post mortemvs.-10.6 for 300PD). Results give evidence of apparent impaired peripheric insulin sensitivity associated with high energy grainbased feeding, either during 300d or 150d. Post mortemPost mortem muscle glycolytic ratio during the first 4h post-slaughter differed due to the energy of diets, suggesting possible meat quality implications.