Feeding strategies and ageing time alter calpain system proteins activities and meat quality of Braford steers

CORIA, MARIA SUMAMPA; PIGHIN, DARIO GABRIEL ; GRIGIONI, G.; PALMA, GUSTAVO ADOLFO

Animal Bioscience

Asian-Australasian Association of Animal Production Societies(AAAP)

Año: 2022

2765-0189

Objective: The aim of this study was to evaluate the effect of ageing and feeding strategieson the calpain protease system and meat quality traits in Braford steers.Methods: Thirty Braford steers were employed; 15 animals were supplemented with cornsilage during finishing and 15 were kept only on pasture. Meat quality traits and calpainsystem protein activity were evaluated in longissimus thoracis et lumborum (LTL) steaksaged for 2, 7, 14, and 21 days.Results: Aged meat showed higher pH and calcium content, while Warner Bratzler shearforce (WBSF) decreased to day 21. No interaction between ageing and diet was seen forquality traits. Steers finished with corn silage showed higher values of water holding capacity,WBSF and free calcium, and lower values of pH and cooking loss. Calpain and calpastatinactivities decreased with ageing. Finishing steers on pasture produced higher values of calpainsand lower values of calpastatin activities. The higher values of calpain 1 activity were observedin muscles aged 2 days from pasture finished animals, and the lower activity of the inhibitorin the 21 days aged samples of the same group.Conclusion: These results suggest a diet by ageing interaction in calpains and calpastatinand this interaction impact in Warner Bratzler Shear Force in Braford LTL muscle.