Modelo experimental de endotoxemia. Resultados preliminares

LAIÑO J; ZELAYA H; HARO C; MARQUEZ A; AGÜERO G

Tafí del Valle. Tucumán, Argentina.

Jornada; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán.; 2009

ABT

In animals, the challenge with a bacterial lipopolysaccharide (LPS) leads to pathophysiological changes similar to the septic shock syndrome in humans. The objective was set up a model of endotoxemia in mice induced by LPS to study the inflammatory and haemostatic alterations. BALB/c adult mice were separated in two groups according to the dose of LPS administered by intraperitoneal injection (iip): 0,5mg of LPS/kg of body weight (LPS0,5) and 2,5mg of LPS/kg of body weight (LPS2,5). Control mices were injected with saline solution (C). At different hours postinjection (hpi) samples of plasma, whole blood and tissue were taken up to determine: a) Prothrombin Time (PT); b) Activated Partial Thromboplastin Time (APTT); c) plasmatic Fibrinogen (F); d) Platelets count; e) Total and differential leucocytes count; f) bacterial translocation (TB) to the liver and spleen. Results: the iip of LPS induced decrease of prothrombinic activity (PT C= 100±1,0 %; LPS0,5 6hpi= 74±1,0 %; LPS2,5 6 hpi= 79±0,8 %), APTT prolongation, increase of F (C=280±21,9mg/dL; LPS0,5 12hpi=364±0,0mg/dL; LPS2,5 12hpi=393±0,0mg/dL), decrease of platelets and leucocytes count, and passage of bacteria to liver and spleen. Conclusion:the different doses of LPS have similar behavior. Also it showed haemostatic alterations accompanied with passage of bacteria to liver and spleen. However, it would be appropriate the evaluation of other dose of LPS to obtain an experimental model with higher haemostatic alterations.