Influence of Lactic Acid Bacteria in Serum Leptin Secretion in Cronic Mild Caloric Restrisction Mice Model

GAUFFIN CANO PAOLA; VALDEZ CECILIA; GULTEMIRIAN MARÍA DE LOURDES; GONZALEZ SILVIA

Egmond aan Zee, Holanda

Simposio; 9th Symposium on Lactic Acid Bacteria; 2008

Federación Europea de Microbiología y Sociedad Holandesa de Microbiología

INFLUENCE OF lACTIC ACID BACTERIA ON serUM leptin SECRETION IN cronic mild CALORIC RESTRICTION MICE MODEL Gauffin Cano1 P, Valdez1 C, Gultemirian1,2 L and González1,2 S 1- Centro de Referencia para Lactobacilos (CERELA) - CONICET, Chacabuco 145 (T4000ILC) Tucumán, Argentina. E-mail: pgauffin@cerela.org.ar 2- Universidad Nacional de Tucumán - Tucumán, Argentina. The objectives of the present study were to establish the time of mild feed deprivation that inhibits secretion of leptin; to assess whether the repletion of diet with lactic acid bacteria resuspended in goat’s milk has influence on serum leptin concentration; and to compare the observed effects with the administration of probiotic fermented goat’s milk (PFM). Weaned Swiss mice were fed with mild caloric restricted diet for 45 or 90 days (CR), and then renourished with goat’s milk during 7 days. After that, animals were refed with ad libitum balanced conventional diet (BCD) and goat’s milk during 7 d followed by 5 d repletion period where animals were fed with BCD and goat’s milk supplemented with a concentration of 108 cfu/animal/day of L. casei CRL 431, or L. rhamnosus ETC14, or probiotic fermented milk (PFM) with L. casei CRL 431 and L. acidophilus CRL730. We determined serum leptin by ELISA test and nutritional parameters. We observed that 45 days induced a significant increase of serum leptin level, while at 90 days a significant descent was observed. We showed that L casei CRL431 did not modify serum leptin levels in both, control and CR groups. Whereas, L. rhamnosus ETC14 and PFM induced a significant increase on serum leptin levels in all groups. These results suggested that the regulation of serum leptin secretion depends on food deprivation period. We demonstrated that LAB administration could modify circulating leptin levels in a strain-dependent way, but it is important if these microorganisms are administered in suspension or in a fermented product, since that additional influence of fermentation metabolites could be involved.