BINDING OF PLANT LECTINS BY DAIRY PROPIONIBACTERIA MAY DECREASE THEIR DELETERIOUS EFFECTS ON COLONIC PHYSIOLGY

ADRIANA PEREZ CHAIA ; GABRIELA ZÁRATE ; GABRIEL SÁEZ

ANAEROBE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2017 vol. 44 p. 58 - 65

1075-9964

Plant lectins are specific carbohydrate-binding proteins widespread in legumes like beans and pulses, seeds, cereals, and many plants used as farm feeds. They are highly resistant to cooking and digestion, reaching to the intestinal lumen and/or blood circulation with biological activity. As result, a series of harmful local and systemic reactions are triggered placing these molecules as antinutritive and/or toxic substances. In the gut, specific cell receptors and microbiota may interact with these dietary components leading to changes on intestinal physiology. It has been proposed that probiotic microorganisms with suitable surface glycosidic moieties could bind to dietary lectins favoring their elimination from the intestinal lumen or inhibiting their interaction with epithelial cells. In this work, we assessed in vitro the effects of two representative plant lectins, concanavalin A (Con A) and jacalin (AIL) on proliferation of SW480 colonic adenocarcinoma cell line and metabolic activity of colonic microbiota in the absence or presence of Propionibacterium acidipropionici CRL 1198. Both lectins induced proliferation in a dose-dependent manner of colonic cell line whereas ConA inhibited fermentative activities of colonic microbiota being these effects prevented to different extents by the presence of propionibacteria. The observed effects could be ascribed to binding of lectins by bacterial cells since P. acidipropionici CRL 1198 was unable to metabolize these proteins and its adhesion to colonic cells was reduced after reaction with Con A or AIL. Results suggest that consumption of propionibacteria at the same time than lectins could reduce the incidence of lectins-induced alterations in the gut and may be a tool to protect the intestinal physiology.