CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
artículos
Título:
MODIFICATION BY GLUCOSE OF THE FLOCCULENT PHENOTYPE OF A Kloeckera apiculata WINE STRAIN
Autor/es:
SOSA O. A.; MANCA DE NADRA; FARÍAS M. E.
Revista:
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Referencias:
Año: 2008 vol. 35 p. 851 - 851
ISSN:
1367-5435
Resumen:
Abstract We have evaluated the induction of the Xocculent phenotype of Kloeckera apiculata by glucose mc1 and propose a pathway involved in carbohydrate Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast propose a pathway involved in carbohydrate Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast phenotype of Kloeckera apiculata by glucose mc1 and propose a pathway involved in carbohydrate Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast propose a pathway involved in carbohydrate Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast We have evaluated the induction of the Xocculent phenotype of Kloeckera apiculata by glucose mc1 and propose a pathway involved in carbohydrate Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast propose a pathway involved in carbohydrate Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast Kloeckera apiculata by glucose mc1 and propose a pathway involved in carbohydrate Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast Xocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast ¡1) and the Xocculation percentage was measured. To elucidate the mechanism involved in Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast Xocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l¡1 glucose pulse, the yeast¡1 glucose pulse, the yeast Xocculation percentage arises to 55 and 65%, respectively. The quantity of proteins and the reXocculating capacity of a lectinic protein extract from the yeast cell wall increase as the concentration of glucose pulse was higher. Cycloheximide prevented the glucose-induced Xocculation, while cAMP or 2,4-dinitrophenol increased it 4- and 5-fold, respectively. PKA inhibitor completely prevented the glucose induction Xocculation. The Xocculent phenotype of K. apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, cAMP or 2,4-dinitrophenol increased it 4- and 5-fold, respectively. PKA inhibitor completely prevented the glucose induction Xocculation. The Xocculent phenotype of K. apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, lectinic protein extract from the yeast cell wall increase as the concentration of glucose pulse was higher. Cycloheximide prevented the glucose-induced Xocculation, while cAMP or 2,4-dinitrophenol increased it 4- and 5-fold, respectively. PKA inhibitor completely prevented the glucose induction Xocculation. The Xocculent phenotype of K. apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, cAMP or 2,4-dinitrophenol increased it 4- and 5-fold, respectively. PKA inhibitor completely prevented the glucose induction Xocculation. The Xocculent phenotype of K. apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, The quantity of proteins and the reXocculating capacity of a lectinic protein extract from the yeast cell wall increase as the concentration of glucose pulse was higher. Cycloheximide prevented the glucose-induced Xocculation, while cAMP or 2,4-dinitrophenol increased it 4- and 5-fold, respectively. PKA inhibitor completely prevented the glucose induction Xocculation. The Xocculent phenotype of K. apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, seems to imply de novo protein (lectin) synthesis via the PKA transduction pathway. This work contributes to the elucidation of the mechanism involved in Xocculation induction by glucose of a non-Saccharomyces wine yeast, induction by glucose of a non-Saccharomyces wine yeast, cAMP or 2,4-dinitrophenol increased it 4- and 5-fold, respectively. PKA inhibitor completely prevented the glucose induction Xocculation. The Xocculent phenotype of K. apiculata mc1 was induced by glucose and the mechanism