Bile effect on three growth parameters of porcine origin Lactobacilli

BERTOZZI, E.; FRIZZO, L.S.; SOTO, L.P.; ZBRUN, M.V.; SIGNORINI, M.L.; SEQUEIRA, G.; ROSMINI M.R.

San Miguel de Tucumán, Tucumán, Argentina

Simposio; III Simposio Internacional de II Bacterias Lácticas. Segundo Encuentro de la Red Argentina de Bacterias Lácticas (Red-BAL); 2009

CERELA-CONICET

The bacteria probióticas can promote the health if they come to the small and large intestines without loss of viability. The bile represents the major challenge to survive and subsequently to colonize the gastrointestinal tract on the part of the microorganisms, indispensable need in order that these realize your o his action probiótica. The levels of bile vary in the intestine in large measure to throughout the day representing a barrier of importance to the microbial growth. This work was intended to know the effect of various concentrations of bile on three parameters of growth in 12 strains of lactobacilli of porcine origin. Studied the maximum growth, the duration of the stage lag and the time of duplication bacterial to determine the behavior of the strains and select under these criteria the best exponents who shall constitute a inoculo probiotic. Strains used were Lactobacillus johnsonii DSPV 001C; Lactobacillus reuteri DSPV 002C; Lactobacillus agilis DSPV 003C; Lactobacillus reuteri DSPV 004C; Lactobacillus agilis DSPV 005C; Lactobacillus ruminis DSPV 006C; Lactobacillus reuteri DSPV 008C; Lactobacillus agilis DSPV 009C; Lactobacillus johnsonii DSPV 010C; Lactobacillus fermentum DSPV 012C; Lactobacillus agilis DSPV 013C y Lactobacillus salivarius DSPV 014C. All are members of the microbiota of pigs from the zone of influence of the Faculty of Veterinary Medicine. For the determinations were used microplates ELISA. In each cavity of the microplates placed 240 ml half MRS (Britain) with concentrations of bile of 0 % (control), 1% 2% 3% 4% 5% 6% and 7 %. Were planted by tripled, and were incubated at 37 ºC. The absorbance was measured at a wavelength of 650 nm to determine the curve of microbial growth. To quantify the strains used a model Logarithmic regression. The results were processed by the program MicroFit© v1.0 to obtain parameters studied. Higher values of maximum growth we showed the strains DSPV 001C, DSPV 002C, DSPV 004C, 006C DSPV and DSPV 010C in the concentrations of 1 % to 6 % of  bile. While at the same mergers, the lower performance was lactobacillus DSPV 005C. The duration of the stage lag was further reduced to the lactobacillus DSPV 006C with significant differences regarding the other microorganisms evaluated in the concentrations of  2 % to 6 % of bile. The times longer for phase lag, in all the concentrations studied, were to the strains DSPV 010C and 001C. The bacterial duplication was highest in the strains DSPV 001C, 008C and 014C in the percentages of bile from 1% to 5 %. From the studied we can determine that Lactobacillus johnsonii DSPV 001C and Lactobacillus ruminis DSPV 006C are the two strains with characteristics more advantageous because of their greater capacity to grow to various concentrations of bile, some of them much higher than what found physiologically in the intestine of pigs.