Preliminary study of lactic acid bacteria in avian blood from slaughterhouses for use as biopreservative

ZBRUN, M.V.; WELSCHEN, N.; SOTO, L.P.; SIGNORINI, M.L.; ROSMINI, M.R.; FRIZZO, L.S.

San Miguel de Tucumán, Tucumán, Argentina

Simposio; III Simposio Internacional de II Bacterias Lácticas. Segundo Encuentro de la Red Argentina de Bacterias Lácticas (Red-BAL); 2009

CERELA-CONICET

Blood is a raw material rich in proteins of high biological value which can be use by a variety of industries. In addition, blood has always been a problem for slaughterhouses since it generates a high-contaminating residual flow, due to its high biological oxygen demand. The main problem with this product is the microbiological quality because it is collected using open systems of collection. One possibility to keep the stability of blood could be through biopreservative culture, using the capacity of the indigenous microbiota to inhibit or control the growth of spoilage or pathogenic microorganisms. The aim of this study was to isolate lactic acid bacteria from avian blood from slaughterhouses for use as biopreservative. Eight blood samples from two poultry slaughterhouses were analyzed microbiologically. The blood was collected in sterile containers during slaughter. Of each sample were made dilutions in Ringer ¼ for count and isolate of lactic acid bacteria (LAB). MRS agar and LAMBAV agar were used in this study. The culture was done in agar surface and incubated at 37°C for 48 h in anaerobiosis. From each plate, 3-4 colonies macroscopically different, were isolated in MRS broth and incubated at 37°C for 24 h. Phenotypic and genotypic tests were carried out to study the different isolates. Bacteria morphology (after Gram staining) was observed, catalase, homo-heterofermentation and arginine hydrolysis tests, were performed. Furthermore, DNA extraction and amplification of 16S rDNA gene, was carried out. The amplified product of 1500pb was digested with three restriction enzymes: Hinf I, Msp I and Hae III. LAB population found in the avian blood from slaughterhouse was 4.94 log CFU/ml in MRS and 3.95 log CFU/ml in LAMBAV. Ninety-eight strains were isolated, 86 rods and 12 cocci. The combination of phenotypic and genotypic tests identified 26 different patterns, which are being sequenced for identification. These preliminary results showed a high count of LAB and a significant diversity in this population in the avian blood from slaughterhouses. From the LAB isolates those with biopreservative properties will be select with the aim to reduce the processing cost, improving the microbiolgical characterisitics of avian blood using a simple and low cost technology.