Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis

BLAJMAN J.E.; ZBRUN, M.V.; SIGNORINI, M.L.; ZIMMERMANN, J.A.; ROSSLER, E.; BERISVIL, A.P.; ROMERO SCHARPEN, A.; ASTESANA, D.M.; SOTO, L.P.; FRIZZO, L.S.

ANIMAL PRODUCTION SCIENCE

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2017 vol. 57 p. 458 - 465

1836-5787

Understandingof the intestinal microbiota is crucial to enhance intestinal health andperformance parameters in animals. A more exhaustive research of the intestinalmicrobiota of broilers could be of interest to implement appropriateintervention measures. The aim of the present study was to investigate thedevelopment of the predominant cecal microbiota in broilers that were fed aLactobacillus salivarius DSPV 001P strain during a complete rearing using denaturinggradient gel electrophoresis (DGGE). Bacterial DNA from cecal samples of 24broilers at different ages were amplified by PCR and analysed by DGGE. A totalof 35 DGGE products were excised and sequenced. Distinctive differences inbacterial communities were observed in the caecum as broilers age. At earlystages, identified bacteria within the caecum of broilers were predominantlyClostridium-related species. Also, some sequences had the closest match to the genusEscherichia/Shigella. Furthermore, the caecum was a reservoir rich inuncultured bacteria. The major difference observed in our study was an increaseof potentially beneficial Lactobacillus at Day 45. These results may beattributed to modulation of the microbiota by the probiotic supplementation.The obtained data could be relevant for future studies related to the influenceof the microbiota resulting from probiotic supplementation on the performanceand the immunological parameters of broilers.