An overview of peroxidation reactions using liposomes as model systems and analytical methods as monitoring tools

FAROUX, J.M.; URETA, M.M.; TYMCZYSZYN, E.E.; GÓMEZ-ZAVAGLIA, A.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Año: 2020 vol. 195

0927-7765

Phospholipids are building blocks of biologicalmembranes having a key role in cellular functionality. The presence ofunsaturated fatty acids in their conformation makes them prompt to oxidationreactions, leading to dysfunctions of living cells or to instability of lipidcontaining food products. For the understanding of these biochemical reactions,liposomes have been widely used as model membrane systems. The aim of thisreview is to gather together the latest advances on the understanding onlipids´ peroxidation, using liposomes as model systems, including the mainavailable analytical methods to monitor peroxidation reactions, with specialemphasis on Fourier Transform Infrared (FTIR) and Raman spectroscopies. Lipid peroxidation is the most widely studied freeradical chain reaction, which occurs in three steps: initiation, propagationand termination, making difficult to determine peroxidation products.Nevertheless, using liposomes as model membrane systems provides a useful toolto investigate the effects of free radicals. In addition, different analyticalmethods enable the determination of peroxidation primary or secondary productsas well as the decrease of reagents (lipids). In particular, FTIR and Ramanspectroscopies allow the simultaneous determination of peroxidation products ina non-destructive and easy-to-use manner. A quick monitoring of both reagentsand products provides a reliable method for the quality control of industrialproducts or even for diagnostics, thus underlying the strong potential ofvibrational spectroscopic based techniques.