Cryopreservation of Andean potato shoot tip monitored by differential scanning calorimetry

ROQUE BORDA CESAR AUGUSTO ; DIGIGLIO ARIANA; LECOT JAVIER; DELADINO LORENA; SCHNEIDER-TEIXEIRA ALINE

Congreso; XXVIII Congreso de la Asociación latinoamericana de la papa; 2018

At the Potato Genebank of the National Institute of Agricultural Technology (EEA Balcarce - INTA), three potatoes landraces of Solanum tuberosum subsp. andingena maintained under slow growth in vitro conservation, were selected to reinitiated the long term conservation by cryopreservation employing a modified droplet vitrification protocol. The effect of dehydration at different times with plant vitrification solution 2 (PVS2) on the potato landraces was studied. The protocol involves excision of about 1 - 2 mm shoot tips in MS medium from in vitro plants, transferred of shoot tips to loading solution and dehydration in PVS2 for 20 and 30 min before rapid cooling in liquid nitrogen (LN). Samples of cryopreserved shoot tips and cryoprotecting solutions where analyzed under a Differential Scanning Calorimetry (DSC) equipment to investigate their thermal activities and detected ice formation during the droplet-vitrification process. Thermograms evidenced diverse response from the three potato varieties towards cryopreservation according the different dehydration times.