An enzymatic method for determination of formate production by Streptococcus thermophilus

PEREZ, P.; DE ANTONI G; AÑÓN, M.C.

JOURNAL OF DAIRY SCIENCE

AMER DAIRY SCIENCE ASSOC-ADSA

Lugar: Champaign, Illinois; Año: 1990 vol. 73 p. 2697 - 2701

0022-0302

An enzymatic detennination of formate in milk samples using formate dehydrogenase (BC 1.2.1.2) was tested. The replacement of HCl04 by TCA in the deproteinization step lead to faster determinations, and no deproteinization was required for samples in culture medium.The method was applied to study formate production by Streptococcus thermophiIus ATCC 19258 grown in milk. and culture media Results obtained with the method show that formate levels are higher in milk. than in the culture medium.(