Fractionation of wheat, barley and rye prolamins by cation exchange FPLC

CHIRDO, FERNANDO GABRIEL; FOSSATI C.A.; AÑÓN MC

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 1994 vol. 42 p. 2460 - 2465

0021-8561

Wheat, rye, and barley prolamins were separated using optimized elution conditions by cation exchange FPLC on an analytical Mono-S column. Wheat gliadins were resolved into a larger number of peaks than has been obtained hitherto. Most fractions contained a mixture of components as determined by SDS?PAGE and A?PAGE. At least one fraction was obtained for each group of gliadins that contains only one major component with minimal contamination by other components.Only ogliadins occurred in five of the fractions. A preparative S-Sepharose Fast Flow column,run under similar chromatographic conditions, was used to isolate oj-gliadins. This procedure could be used as the first step for the purification of individual gliadin components. Chromatography on the Mono-S column was highly reproducible and showed promise as a means of differentiating wheat cultivars. Furthermore, FPLC was applied to fractionate prolamins from barley and rye.