Thermal denaturation of muscle proteins from male and female squid (Illex argentinus) at different sexual maturation stages. A differential scanning calorimetric study

PAREDI, MARIA E.; TOMÁS. M.C; CRUPKIN, M; AÑON, MARÍA CRISTINA

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 1996 vol. 44 p. 3812 - 3816

0021-8561

The influence of both sex and sexual maturation stage of specimens on the thermal behavior ofmuscle proteins was investigated. Differential scanning calorimetry (DSC) thermograms of wholemuscle of female squids showed four endothermic transitions [peak temperature maximum (Tmax),45.9, 56.8, 67.2, and 79.2 °C]. The DSC thermograms of whole muscle of male squids showed threetransitions (Tmax, 47.9, 56.8, and 79.2 °C). The Tmax of 67.2 °C, present only in female squid muscle,was related to sarcoplasmic proteins. Myosin and paramyosin contributed to the first transition,connective tissue to the second transition, and actin to the last transition. No major differenceswere observed in Tmax values that were related to the sex and sexual maturation stage of specimens.The lowest denaturation enthalpy (¢H) was found in muscle from immature females. Independentlyof the sex and the sexual maturation stage of specimens, no major changes were observed in eitherTmax or ¢H values during frozen storage of squids.