The RNA composition of virus Junin

AÑON, MARÍA CRISTINA; GRAU O; MARTINEZ SEGOVIA ZM

JOURNAL OF VIROLOGY

AMER SOC MICROBIOLOGY

Lugar: Washington; Año: 1976 vol. 18 p. 833 - 838

0022-538X

Junin virus grown in BHK-21 cells was labeled with [3H]uridine and highlypurified by differential and isopycnic centrifugation. The RNAs extracted withphenol and analyzed by polyacrylamide gel electrophoresis were shown to becomposed of four large species (33, 28, 25, and 18S) and three small ones (4, 5,and 5.5S). This pattern was similar to ones already reported for other arenaviruses. However, there was a striking difference when the virus labeling was performed in the presence of actinomycin D. Labeling of viral rRNA was asmuch as 60% of the levels obtained in the absence of the drug under conditions in which cellular rRNA's were inhibited by 95% or more.