Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion

SABBIONES, A.C; NARDO, A; AÑON, MARÍA CRISTINA; SCILINGO, A.A

Journal of Functional Foods

Elsevier SRL

Año: 2016 vol. 20 p. 204 - 214

Amaranth protein isolate was obtained and subjected to simulated gastrointestinal digestionto evaluate its potential antithrombotic activity.The protein isolate did not present fibrinclotting inhibition at the concentrations studied, whereas the hydrolysate (DH% = 51.1 ± 3.8%)exhibited inhibition of fibrin coagulation, showing a dose?response behaviour(IC50 = 0.23 ± 0.02 mg/mL), confirming that the enzymatic treatment was able to release bioactivepeptides from amaranth proteins. A fraction with high antithrombotic activity was obtainedfrom this hydrolysate, and resulted to be three times more potent than its originalsample (IC50 = 0.07 ± 0.01 mg/mL). The absorption of this active fraction was studied withan in vitro peptides transport assay through intestinal epithelium and it was observed thatsome peptides are able to cross the Caco2-TC7 cell monolayer. Potentially bioactive peptideswere found after sequencing them, and informatics tools allowed us to select and locatein their native molecules those peptides prone to inhibit thrombin activity.