INVESTIGADORES
AÑON Maria Cristina
artículos
Título:
Effect of frozen storage on protein denaturation in bovine muscle 1. Myofibrillar ATPase activity and differential scanning calorimetric studies
Autor/es:
J. R. WAGNER; AÑON, M, C
Revista:
BRAZILIAN JOURNAL OF FOOD TECHNOLOGY
Editorial:
.
Referencias:
Año: 1986 vol. 21 p. 9 - 18
ISSN:
1516-7275
Resumen:
The effect of frozen storage on myofibrillar ATPase activity and thermal transitions in
bovine muscle was investigated. Myofibrillar ATPase activity and total enthalpy of
denaturation (AH) decreased with time of storage. The rate of decrease was lower at
-20°C than at -5°C or -10°C. Differences in behaviour during storage of muscle after
fast or slow freezing could be attributed to differences in ice recrystallization. The
observed decreases in area of the first peak seen in the thermograms and Ca2+-myofibrillar
ATPase activity show that the myosin head denaturated progressively during
storage. The myosin tail also denaturated during storage but the thin filament remained
unaltered. Kinetic analysis suggested that the denaturation of the myofibrillar proteins
took place through two consecutive first order reactions; an initial rapid reaction
followed by a slower one.