Probiotic characterization of indigenous lactic acid bacteria isolates from chickens to be used as biocontrol agents in poultry industry

MORETTI, ANA F.; GAMBA, RAÚL; DE ANTONI, GRACIELA; PELÁEZ, ÁNGELA LEÓN; GOLOWCZYC, MARINA A.

JOURNAL OF FOOD PROCESSING AND PRESERVATION

WILEY-BLACKWELL PUBLISHING, INC

Año: 2022

0145-8892

Lactic acid bacteria (LAB) are used as probiotics to improve animal health and production. This study aimed to characterize and assess three LAB, Ligilactobacillus salivarius 1234C, Ligilactobacillus agilis 1235C and Enterococcus durans 12311C, isolated from chicken, as potential probiotic candidates to improve the production parameters of commercial poultry. Resistance to gastrointestinal conditions, growth in alternative culture media, antagonistic activity against Salmonella and mycotoxigenic fungi were investigated. Additionally, the strains were added to poultry feed to evaluate their in vivo effect. The three LAB showed high survival rates after sequential gastric and intestinal conditions, demonstrated bactericidal activity against Salmonella and were able to grow in alternative culture media. Also, ligilactobacilli strains exerted fungicidal activity against mycotoxigenic fungi. In vivo assay showed that dietary supplementation of a multi-microbe probiotic product with 107 CFU/ml significantly increased (p