Effect of antibodies on lactoperoxidase of mammals species

C. VAN NIEUWENHOVE; S.N. GONZÁLEZ; S.SAAD

Tafí del Valle- Tucumán

Jornada; XIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2001

Asociación de Biología de Tucumán

Effect of antibodies on milk  lactoperoxidase of mammals species C. Van Nieuwenhove, S. González, S. Saad   Lactoperoxidase (LPO) is an enzyme present in many exocrine secretions of mammals. Its form part, with the hydrogen peroxide and thiocyanate anion, of named “LPO system”, which has been proved to has antibacterial activity, due to shot-lived oxidation products from the LPO-catalyzed oxidation of thiocyanate. LPO is the most abundant enzyme in bovine milk, where many studies were done. The aim of this work was to evaluate the effect of antibodies anti-bovine LPO on enzyme of milk of seven different species. LPO assays were carried out according to Marshall et al. (1986), modified by Saad de Schoos (1996). The samples were measured before and after incubation for 30 minutes at 37 ºC with mentioned antibodies. Results showed that LPO of all species was inhibited at higher antibody concentration. The lower inhibition was showed in mammals belonging to different order than cow. The immunogenic similarity of LPO could be related to the filogenic of species.