Hydrolysate of β-lactoglobulin by Lactobacillus delbrueckii subsp. bulgaricus CRL 656 suppresses the immunoreactivity of β-lactoglobulin as revealed by in vivo assays

PESCUMA, M.; HÉBERT, E.M.; FONT, G.; SAAVEDRA, L.; MOZZI, F.

INTERNATIONAL DAIRY JOURNAL

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2019

0958-6946

Cows? milkallergy, common in young infants, is often solved by the age of five; however,it can persist in some adults. The in vivo immunomodulatory potential of β-lactoglobulin (BLG) hydrolysatesobtained with Lactobacillus delbrueckii subsp. bulgaricus CRL656 (H656) wasstudied. Sera from mice gavaged with H656 showed lower specific BLG-IgE valuesthan those of allergic mice. Secretion of IL-10, INF-γ and IL-6 was increased and that of IL-4decreased when allergic spleen cells were stimulated with H656. IL-4 secretionwas significantly reduced and concentrations of IL-10, IL-17A and IL-6 wereincreased when H656 was orally administrated. Highest expression of IL-4 andIL-12 was observed for allergic mice and H656 gavaged mice, respectively. H656immunisation had a positive effect on intestinal mucosa. Overall, H656displayed an immunomodulating effect, balancing the allergic Th2 response bystimulating Th1 type cytokine secretion. The hydrolysate might promote oraltolerance towards BLG in infant foods.