INVESTIGADORES
HEBERT Elvira Maria
artículos
Título:
Characterisation of a cell-envelope proteinase of Lactobacillus helveticus
Autor/es:
HEBERT, E.M.; RAYA, R.R.; SAVOY DE GIORI, G.
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Año: 1999 vol. 21 p. 831 - 834
ISSN:
0141-5492
Resumen:
A cell-envelope proteinase from Lactobacillus helveticus CRL 1062 was
characterisated. The enzyme was not released from washed cells in absence of
calcium. It had maximum activity at pH 6.5-7.0 and 42°C. The enzyme hydrolysed
a- and b-caseins
with different degradation
rates. Activity was strongly inhibited by PMSF, suggesting the enzyme is a
serine-type protease.