Specific strains of lactic acid bacteria differentially modulate the profile of adipokines in vitro

FABERSANI E.; ABEIJÓN MUKDSI CLAUDIA; ROSS ROMINA; MEDINA ROXANA; GONZÁLEZ SILVIA NELINA; GAUFFIN CANO PAOLA

Frontiers in Immunology

Frontiers Media S.A. - Haruki Kitazawa, Tohoku University

Lugar: Tohoku; Año: 2017 vol. 8

Obesity induces local/systemic inflammation accompanied by increases in macrophageinfiltration into adipose tissue and production of inflammatory cytokines, chemokines,and hormones. Previous studies have shown that probiotics could improve the intestinaldysbiosis induced by metabolic diseases such as obesity, diabetes, and metabolic syndrome.Microorganisms could (directly or indirectly) affect adipokine levels due to theircapacity to induce translocation of several intestinal microbial antigens into systemiccirculation, which could lead to metabolic endotoxemia or produce immunomodulationin different organs. The aim of the present study was to select non-inflammatory lacticacid bacteria (LAB) strains with the capacity to modulate adipokine secretion by theadipose tissue. We wish to elucidate the role of potential probiotic strains in the regulationof the cross talking between immune cells such as macrophages and adiposecells. Mouse macrophage cell line RAW 264.7 was used for evaluating the ability of 14LAB strains to induce cytokine production. The LAB strains were chosen based on theirpreviously studied beneficial properties in health. Then, in murine adipocyte culture andmacrophage?adipocyte coculture, we determined the ability of these strains to inducecytokines and leptin secretion. Tumor necrosis factor alpha, interleukin 6 (IL-6), IL-10,monocyte chemoattractant protein-1, and leptin levels were measured in cell supernatants.We also performed the detection and quantification of leptin receptor (Ob-Rb)expression in macrophage cell lines stimulated by these LAB strains. Differential secretionprofile of cytokines in macrophage cells induced by LAB strains was observed. Also,the levels of Ob-Rb expression diverged among different LAB strains. In LAB-stimulatedcoculture cells (adipocytes and macrophages), we observed differential production ofleptin and cytokines. Furthermore, we detected lower production levels in single culturethan cocultured cells. The principal component analysis showed an association betweenthe four clusters of strains established according to their inflammatory profiles and leptinadipocyte production and leptin receptor expression in macrophages. We conclude thatcoculture is the most appropriate system for selecting strains with the ability to modulateadipokine secretion. The use of microorganisms with low and medium inflammatoryproperties and ability to modulate leptin levels could be a strategy for the treatment ofsome metabolic diseases associated with dysregulation of immune response.