LIPID PEROXIDATION, OXIDATIVE STRESS, PROTEIN OXIDATION AND ANTIOXIDANT CAPACITY DURING SKELETAL MUSCLE REGENERATION IN VIVO

SCARLATA, E.; DESCALZO ADRIANA; INSANI M,; ROSSETTI, LUCIANA; PENSEL, N.A.

FREE RADICAL BIOLOGY AND MEDICINE

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2004 vol. 36

0891-5849

Reactive oxygen species (ROS) are involved in physiologicalfunctions. Skeletal muscle has a remarkable ability toregenerate after bupivacaine inyection. Our aim was todeterminate the levels of lipid peroxidation (LP), oxidativestress (OS), protein oxidation (PO) and antioxidant capacity(AC) during the skeletal muscle regeneration (SMR)in vivo. Bupivacaine was injected in the anterior tibialmuscle of adult female Wistar rats. Muscles were removedat 0, 1, 3, 5, 7 and 15 days post-injection, and LP, OS, POand AC were determined by the thiobarbituric acidreactive substances (TBARS) assay, chemiluminescencein vivo, levels of carbonyl group and the ferric reducingability of plasma (FRAP) assay, respectively. The SMR wasfollowed by electron microscopy. Significant increases ofLP were observed on day 7 respect to control (39.8 F 6.6vs 19.7 F 4.5 nmol TBARS/gWT); PO increased until amaximum on day 7 compared to control (2.02 F 0.94 vs0.948 F 0.15 nmol/mg protein). A significant increase ofOS on day 7 compared to control (311 F 50 vs 125 F 31cps/cm2) was observed. AC significant increased on days3 to 5 compared to control (47 F 11.1, 46 F 11.9 and22 F 1.1mM FRAP/gWT, respectively), and decreased inday 7 (29 F 1.7, p < 0.05). There were significant relationshipbetween LP and OS (r = 0.89), LP and PO (r =0.53), PO and OS (r = 0.58) and, AC and OS (r =0.83), AC and LP (r = 0.75). These results suggestthat ROS could have a beneficial effect during SMR.