Complete genome sequencing of Lactobacillus plantarum UNQLp11 isolated from a Patagonian Pinot Noir wine

IGLESIAS N.G; BRIZUELA N.S; TYMCZYSZYN EE; HOLLMAN A; VALDÉS LA HENS D; SEMORILE L; BRAVO FERRADA B.M

SOUTH AFRICAN JOURNAL OF ENOLOGY AND VITICULTURE

SOUTH AFRICAN SOC ENOLOGY & VITICULTURE-SASEV

Año: 2020 vol. 41 p. 197 - 209

0253-939X

The Lactobacillus plantarum UNQLp11 strain was isolated from a Patagonian Pinot noir wine, at the oldest commercial winery (110 years old) in General Roca, North Patagonia, Argentina, and has demonstrated its ability to survive during winemaking processes and successfully carry out the malolactic fermentation .The aim of this work was to obtain the whole assembled genome of the UNQLp11 strain, analyzing its architecture and the possible functions of the predicted genes with the enological properties of this strain. The genome size is 3,534,932 bp, with a mean GC content of 44.2%, 3,412 CDS, 80 transposons and, 148 tandem repeats. A comparison between genome size and gene content in 14 Lb. plantarum strains from different origins was performed, and UNQLp11 exhibited the largest size. The in silico genome-wide analysis allowed us to confirm the existence of genes encoding enzymes involved in the synthesis of several metabolites of enological interest, in addition to bacteriocins and expolysaccharides. Furthermore, it is possible to speculate on this strain?s adaptation to different environments, being able to use diverse substrates for its growth. All these features suggest the potential of UNQLp11 as a good starter culture for MLF.