Quantitative comparison of phytochemical profile, antioxidant, and anti-inflammatory properties of blackberry fruits adapted to Argentina

FRANCO VAN DE VELDE; MARY H. GRACE; DEBORA ESPOSITO; MARÍA ÉLIDA PIROVANI; MARY ANN LILA

JOURNAL OF FOOD COMPOSITION AND ANALYSIS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2016 vol. 47 p. 82 - 91

0889-1575

The phytochemical profile by HPLC-TOF-MS ofthree blackberry cultivars (?Jumbo?, ?Black Satin? and ?Dirksen?), adapted tothe central-east of Argentina, was determined. The antioxidant capacity by DPPHand FRAP assays, and the effect of the blackberry extracts onlipopolysaccharide (LPS)-induced nitric oxide (NO) production, reactive oxygenspecies (ROS) production, and biomarkers of inflammation were also evaluated.?Dirksen? fruits exhibited the highest vitamin C content (24 and 14% higherthan values found in ?Black Satin? and ?Jumbo?, respectively). However, ?Jumbo? and ?Black Satin? fruits presentedhigher total phenolic contents (more than 15%) than ?Dirksen?. Cyanidin-3-O-glucoside was the mainpolyphenolic compound quantified in all samples. ?Jumbo? and ?BlackSatin? cultivars exhibited higherantioxidant capacity, and significantly reduced the release of ROS. ThemRNA expression levels of cyclooxygenase-2 (Cox-2) and interleukin-6 (IL-6)were reduced consistently (more than 30%) byextracts of both cultivars, and showed slight suppressionson NO production. However, effective inhibition inthe gene expression of interleukin-1β(IL-1β) and nitric oxide synthase(iNOS) was not observed by any of extracts. These results suggest the potentialof blackberries cultivars `Black Satin? and ?Jumbo? to contribute to a healthydiet, based on their higher polyphenol content, providing higher antioxidantand anti-inflammatory properties.