SINGLE NUCLEOTIDE POLYMORPHISMS IN MMP-1 AND THE RELATION WITH BEEF FATTY ACIDS PROFILE

CHRISTENSEN SARA; POUZO LAURA; LATORRE M. EMILIA; MONTEAVARO CRISTINA; PAVAN ENRIQUE; PURSLOW PETER

Cork

Congreso; 3rd International Congress of Meat Science and Technology to be held in Cork, Ireland, on the 13th-18th August, 2017; 2017

Matrix metalloproeinas -1 (MMP-1) is an extracellular collagenase that is also know to have cell signaling effects controlling adipogenesis. Previous research indicates that single nucleotide polymorphisms (SNPs) in MMP-1 can affect the fatty acid profile and fat content of beef muscles. The aim of the research is to determine if the SNPs ss77831914 and ss77831924 in MMP-1 have effects on the development of fatty acids in meat in comparison to the effect of diet. Thirty (30) Angus cattle were raised on three diets and the Tetra primers ARMS-PCR was the technique used to identify the single nucleotide polymorphisms. Fatty acid methyl esters were analyzed using a CLARUS 500 gas chromatograph. In our study, no correlation were found between the SNPs ss77831914 and ss77831924 and the fatty acid profile but a higher amount of fatty acids was found in homozygotic animals (GG genotype) for the SNP ss77831914 than for heterzygotic (CG) animals. However, this effect was insignificant compared to the effect of diet. We conclude that SNPs in MMP-1 may affect the intramuscular fat content of beef Longissimus thoracis muscle in the absence of dietary effects