High molecular weight chitosan based particles for insulin encapsulation obtained via nanospray technology

PRUDKIN-SILVA, CECILIA; MARTÍNEZ, JIMENA H; MAZZOBRE, FLORENCIA; QUIROZ-REYES, CINTHYA; SAN-JUAN, ERWIN; SAN-MARTÍN, EDUARDO; PÉREZ, OSCAR E.

DRYING TECHNOLOGY

TAYLOR & FRANCIS INC

Lugar: v.40 no.2 Find all articles in: Drying technology 2022 v.40 no.2 pp. 430-445; Año: 2020 vol. 40 p. 430 - 445

0737-3937

The objective of this work was to obtain Chitosan (CS) based particles for Insulin (INS) encapsulation, via nanospray drying of a feeding solution containing equal amounts of both components (0.1% w/v total solids content). The process was performed at pH 3 which is out of the range for electrostatic interactions to occur. The analysis involved the nanoparticles (NP) characterization in the solution before drying (pH 3) by dynamic light scattering (DLS) and after re-hydration at different pHs (3< pH < 11). The dried product was characterized by Fourier-transform spectroscopy (FTIR), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). FTIR allowed detecting the chemical groups involved in INS-CS interactions. The encapsulation efficiency of the glassy NP was 62.3 ± 0.32% as determined by HPLC. Upon powder re-hydration, NP of diameter