ALKALINE PHOSPHATASE IN SHRIMP Artemesia longinaris: RESPONSE TO VITAMIN D IN DIET.

FENUCCI, JORGE LINO; PEREIRA, NAIR DE LOS ANGELES; FERNÁNDEZ GIMENEZ, ANALIA VERÓNICA; HARÁN NORA SELMA

Natal

Congreso; World Aquaculture Society 2011; 2011

World Aquaculture Society

The shrimp Artemesia longinaris is abundant penaeid species in the Argentine coastal waters. As the availability of this species undergoes yearly fluctuations, it is important to establish the feasibility of culturing. Diet quality is an important condition for a successful culture of crustaceans. Numerous studies have revealed that fat-soluble vitamins are essential in most animals for normal health and life functions. Vitamin D plays important role in calcium metabolism, lack of this nutrient was correlated with a variation in alkaline phosphatase activity in crustaceans. The alkaline phosphatase activity in midgut gland and haemocytes of shrimp fed with diets containing different levels of vitamin D was studied in the present work. Five semi purified diets (vitamin free casein, manioc starch, squid protein concentrate and vitamin free fish oil) were designed containing 0 (D0); 8,000 (D8), 15,000 (D15); 30,000 (D30) and 40,000 (D40) UI vitamin D3/kg diet. Shrimps were obtained from a commercial fisherman and placed in 150 l glass aquaria (33‰ salinity, 20°C, pH 7, 13:11 h photoperiod). Each diet was tested in three replicate groups of 8 shrimps/m2 during 6 weeks. At the end of trial all specimens were placed on ice and midgut glands and haemolymph were extracted. Wild shrimp (Di) were use as control. Alkaline phosphatase activity was determined in the protein extract of midgut gland and haemocytes (HLS) using p-nitrophenylphosphate (p-NPP) (Sigma N4645) as substrate. The present study shows the occurrence of different alkaline phosphatase activities in the midgut gland and haemocytes of Artemesia longinaris in response to varying levels of Vitamin D in diet. Shiau and Hwang (1994) reported in that absence and levels above 0.1 mg/kg vitamin D in shrimp diet, correlates with a variation in activity of the alkaline phosphatase in Penaeus monodon. Alkaline phosphatase activities obtained for shrimp fed D8; D15 and D30 were nearest to the value obtained for wild shrimp, whereas the other diets had higher values which can be attributed to nutritional stress. Mazorra et al. [32] pointed out that alkaline phosphatase activity can be used as an indicator of biochemistry stress. These suggest that alkaline phosphatase can be use as stress bioindicator; given that deficiency and hypervitaminosis of Vitamin D in A. longinaris increased the enzyme activity.