Development and characterization of nano-vehicles based on argentinian Chitosan:promising carriers for the encapsulation and protection of Resveratrol

FLORENCIA BUOSI; M ALEJANDRA CASTAÑEDA CASTAÑA; KARINA D MARTÍNEZ; CAROLINA DISANTO; ANA PAULA DOMÍNGUEZ-RUBIO; AGUSTINA ALAIMO; OSCAR E PÉREZ

Buenos Aires

Congreso; Frontiers in Biosciences 3; 2018

Instituto de Investigación en Biomedicina de Buenos Aires- Partner of the Max Planck Society

Resveratrol (RSV) is a natural polyphenol which is rich in a variety of plants and fruits, such as grapes, peanut and mulberry. This compound has been shown of possessing multiple pharmacological and healthy benefits, including anti-oxidation/inflammation. However, the biomedical applications of RSV remain limited due to its short biological half-life, labile properties, and rapid metabolism and elimination.Chitosan (Chi) is a naturally occurring and abundantly available polysaccharide extensively studied for drug delivery systems due to the biological properties such as its relative non toxicity, biocompatibility, biodegradability, cationic properties and mucoadhesive characteristics. Chi and tripolyphosphate-sodium (TPP, cross-linking agent) can form biocompatible, cross-linked spheres through electrostatic interactions that can be efficiently employed in bioactives delivery. On the other hand, treatment of diseases of the posterior segment of the eye, such as Age-related Macular Degeneration (AMD), requires novel drug delivery systems that can overcome the many barriers for efficacious delivery of therapeutic drugs. This challenge has prompted us to develop an innovative Chi-TPP nanosystem for the encapsulation, protection and controlled release of RSV and its subsequent evaluation in an in vitro model of AMD. Results: colloidal particles of Chi-TPP (3mg/ml-0.25mg/ml) were obtained by ionic gelation. The particle size distribution and the ζpotential were recorded by dynamic light scattering (DLS, Malvern Z equipment). The peaks of the majority populations were located at 50 and 500 nm. A decrease in the maximum fluorescence peak of RSV was quantified by interaction with Chi. In addition, the protective effect of Chi against UV (254nm, 15W, 90 min)-induced photodegradation of RSV was determined by spectrophotometry (λem = 306nm; POLARstarOmega).Based on our findings, we envision that the nanoencapsulation with Chi-TPP nanospheres could be potential carrier for RSV, opening the possibility of its application for ophthalmic use.