COMPARATIVE STUDY OF High intensity ultrasound effects on food proteins functionality

CAROLINA ARZENI; KARINA DAFNE MARTÍNEZ; PAULA ZEMA; AYELEN ARIAS; OSCAR E. PÉREZ; ANA M. R. PILOSOF

JOURNAL OF FOOD ENGINEERING

ELSEVIER SCI LTD

Año: 2011 vol. 108 p. 463 - 472

0260-8774

ABSTRACT The objective of this work was to comparatively explore the impact of high intensity ultrasound (HIUS) on the functionality of some of the most used food proteins at the industrial level: whey protein concentrate (WPC), soy protein isolate (500 E) and egg white protein (EW) Solutions at 10%, w/w at their respective natural pH were submitted to HIUS treatment for 20 min, in Sonics VCX 750 equipment. The operating conditions were: 20 kHz and 20% of amplitude. Before and after the HIUS treatment, the size of protein particles was measured by light scattering using a Mastersizer 2000. The amount of sulfhydryl groups was determined with Ellman´s reagent and the surface hydrophobicity by a fluorescence technique. The effects of HIUS on samples viscosity were determined in a Brookfield DV-II viscometer. The evolution of the elastic (G´) and viscous (G´´) moduli as well as tan  were registered upon time and temperature in a Phaar Physica MCR 300 controlled stress rheometer. In general, HIUS promoted a decrease in the consistency index of all protein solutions, mainly of soybean isolate. The gelation performance of EW was not modified by HIUS. However, WPC presented a higher elastic character, but 500 E did not show changes upon heating, as it was already denatured before HIUS treatment. The size of aggregates suffered an overall reduction for WPC and 500E, but a slight increase for EW. Sulfhydryl content was unchanged for all proteins after HIUS application but surface hydrophobicity was greatly increased after treatment for all proteins. HIUS affected the studied functional properties differently depending on the size and nature of the protein. This technology could be used to obtain improved functional properties in some protein samples. Keywords: Whey Protein Concentrate, Soy and Egg white Proteins, High intensity Ultrasound, Viscosity, Dynamic Rheology, Gelling Properties.