Extraction and purification of enzymes from the Southern eagle ray (Myliobatis goodei) by-products and their compatibility with detergents: A practical approach towards circular economy

DANIELA LAMAS; AGUEDA MASSA

IRANIAN JOURNAL OF FISHERIES SCIENCES

IRANIAN FISHERIES RESEARCH ORGANIZATION

Año: 2023 vol. 22 p. 853 - 870

1562-2916

Nowadays, the need to simultaneously achieve the valorization and recovery of fishingwaste is critical for addressing environmental challenges and promoting resourcesustainability. Fishing waste hosts enzymes that hold the potential for extraction andutilization in various industries. In this sense, the aim of this work was to extract andpurify enzymes from the gastrointestinal tract of Myliobatis goodei using low-costprocesses. The proteolytic and lipolytic activities of the extracted enzymes were alsoinvestigated. In addition, the detergent compatibility of the purified enzyme extract wasevaluated to determine its potential application as an additive in laundry soaps. The crudeextract was obtained by homogenization of 100g gastrointestinal tract in buffer Tris-HCl(pH=8.0). Then, it was precipitated with ammonium sulfate and purified by filtration.Finally, it was subjected to centrifugation at 10,000 ×g for 30 min at 4°C in an ultrafiltersmembrane with a 100 kDa cut-off. This purification protocol showed good performancefor proteases and lipases as their activity was recovered at each step. The proteolyticactivity was assayed using azocasein as substrate at pH 8.0 and 40°C, while trypsinactivity was analyzed against the specific substrate benzoyl-arginine-p-nitroanilide. Thelipolytic activity was carried out by p-nitrophenol production through the reaction of pnitrophenylacetate dissolved in 2-propanol at pH 7.0 and 37°C. The compatibility of theenzyme activity with some commercial detergents was evaluated. Additionally,proteolytic and lipolytic activities were tested on spiruline and soybean oil stains andshowed a strong ability to remove them from cotton fabrics. Due to its promisingproperties, the purified enzymes isolated from the gastrointestinal tract of M. goodei maybe considered as a potential effective active ingredient for its use in the detergentsindustry.