CIDCA   05380
CENTRO DE INVESTIGACION Y DESARROLLO EN CRIOTECNOLOGIA DE ALIMENTOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Use of a food allergy mouse model to study the in vivo and in vitro cross-reactivity between cows milk and soybean proteins
Autor/es:
SMALDINI, PAOLA; CURCIARELLO R; CANDREVA A; FOSSATI C. ALBERTO; PETRUCCELLI S; DOCENA G
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Congreso; First French-Argentine Immunology Congress, FAIC 2010; 2010
Institución organizadora:
Sociedad Argentina de Inmunología, Sociedad Francesa de Inmunología
Resumen:
Treatment of
food allergy consists in allergen avoidance and food replacement. Cow´s milk
(CM) is frequently substituted for soy (S)-containing formulae, however it is
not tolerated in some patients even if they were not previously exposed to soy proteins.
Our group has identified caseins and soy globulins as the cross-reactive
allergens. They were obtained as recombinant proteins and cross-reactive epitopes
were mapped.
The aim of
this work was to employ a validated food allergy mouse model to study the in vivo and in vitro cross-allergenicity between CM and S.
BALB/c mice
were intragastrically sensitized with CM proteins (CMP) and cholera toxin (CT)
as a Th2 mucosal adyuvant. Control group consisted of mice exposed to CMP or
CT. An oral challenge was performed with CMP or S, and 15-30 minutes afterwards
clinical signs were recorded (clinical score). Cutaneous tests were performed,
specific antibody (IgE, IgG1 and IgG2a) and histamine serum levels were
quantified, antigen-specific proliferation of splenocytes was analyzed and
cytokine production was measured.
Sensitized
animals showed higher clinical scores than control mice, and both CMP and S
induced symptoms. Specific IgE and IgG1 antibodies were increased in sensitized
mice, while IgG2a was suppressed. Histamine was increased and splenocytes were
stimulated with CMP and S extracts. IL-5 (CMP: 132 ± 64 pg/ml vs. nd, S: 29± 11
vs 7±3 pg/ml, sensitized vs control group resp.) was also increased, while γ-IFN
was basal (CMP: 80±16 vs. 26±13 pg/ml, S: 126±52 vs. 390±170 pg/ml sensitized
vs control group resp.). Cutaneous tests were positive for CMP and soy.
In
conclusion, intragastric sensitization with CMP induced a specific Th2-bias
activation of the gut mucosa immune system that also reacted with soy proteins.
Cross-allergenicity was also evidenced by elicitation of clinical signs and
cutaneous tests. These results
may constitute the experimental basis to develop a mucosal vaccine.