Development and storage studies of high density macrocapsules containing Lactobacillus spp. strains as nutritional supplement in young calves

ASTESANA, D.M.; ZIMMERMANN, J.A.; FRIZZO, L.S.; ZBRUN, M.V.; BLAJMAN, J.E.; BERISVIL, A.P.; ROMERO SCHARPEN, A.; SIGNORINI, M.L.; ROSMINI, M.R.; SOTO, L.P.

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2018

0325-7541

The aim of this study was to evaluate different production methodologies of probioticmacrocapsules with high bacterial densities destined to lactating calves. Three typesof capsules containing Lactobacillus casei DSPV318T and Lactobacillus plantarum DSPV354Twere prepared from an overnight culture in whey medium: (1) mixing the culture with calciumalginate and then, reincubating the capsules in whey (RC); (2) concentrating the biomass bycentrifugation and mixing the pellet with calcium alginate (CC) at different concentrations withrespect to the initial culture (5X and 12.5X); (3) CC with cryoprotectants: whey permeate (Per)and glycerol (Gly). Chitosan coating was evaluated. Capsules were freeze-dried and viabilitywas assessed before freezing, after freeze-drying and every two weeks for 84 days of storageat room temperature, 4 ◦C and −20 ◦C. CC showed higher cell densities than RC. Storagetemperature affected viability: greater viability at lower temperature. Moreover, the effect oftemperature was influenced by other factors, such as capsule coating, culture neutralizationand cryoprotectants. Coating improved viability at room temperature; however no effect wasobserved at 4 ◦C. Culture neutralization allowed greater survival during storage. Cryoprotectantsimproved viability during freezing, but they also generated a positive or negative effectdepending on storage temperature. The best results were: at refrigeration Gly12.5X exhibitedcounts above 109 CFU/capsule until day 70 and Per12.5X until day 56 of storage and at −20 ◦Ccounts above 109 CFU/capsule until the end of the study (84 days). A 109 CFU capsule is thedaily dose per calf which would facilitate the administration of this probiotic inoculum to fieldanimals.